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KMID : 1156220110370040279
Journal of Environmental Health Sciences
2011 Volume.37 No. 4 p.279 ~ p.288
Chemical and Microbiological Hazard Analysis of Floor-Settled Dust in Elementary School Classrooms in Korea
Kim Ji-Young

Jung Se-Young
Kim Soo-Jung
Kim Jin-Ah
Si Ji-Yeon
Jo Yeon-Woo
Zoh Kyung-Duk
Ko Gwang-Pyo
Abstract
Objectives: This study investigated the levels and components of floor-settled dust in two elementary schools located at different sites (one near the Shihwa industrial complex and the other in a rural area) in order to evaluate the amounts of trace metal elements (As, Cd, Co, Cr, Cu, Ni, Pb and Zn) and microorganisms.

Methods: Over twenty settled-dust samples were collected from the two elementary schools. Trace metal elements were extracted from the dust using hydrochloric acid and nitric acid, and the amounts were measured by ICP-OES.
Microbiological analysis was performed by bacterial culturing using R2A medium and denaturing gradient gel electrophoresis (DGGE).

Results: The results showed that the amounts of three metal elements (Cr, Pb, and Zn) were significantly different between the schools (¥á=0.05, p<0.04). In addition, microbial communities in each school were highly correlated with one another. Among the identified microorganisms, a number of potentially opportunistic microorganisms, including antibiotic-resistant bacteria such as Acinetobacter baumannii, were found.

Conclusions: This study will provide preliminary data for assessing levels and types of chemical and microbiological agents in elementary schools and for further evaluating human health risks associated with the agents.
KEYWORD
Bacterial antibiotic resistance, Denaturing gradient gel electrophoresis, Dust, Heavy metals, Shihwa industrial complex
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