KMID : 1156220110370040279
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Journal of Environmental Health Sciences 2011 Volume.37 No. 4 p.279 ~ p.288
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Chemical and Microbiological Hazard Analysis of Floor-Settled Dust in Elementary School Classrooms in Korea
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Kim Ji-Young
Jung Se-Young Kim Soo-Jung Kim Jin-Ah Si Ji-Yeon Jo Yeon-Woo Zoh Kyung-Duk Ko Gwang-Pyo
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Abstract
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Objectives: This study investigated the levels and components of floor-settled dust in two elementary schools located at different sites (one near the Shihwa industrial complex and the other in a rural area) in order to evaluate the amounts of trace metal elements (As, Cd, Co, Cr, Cu, Ni, Pb and Zn) and microorganisms.
Methods: Over twenty settled-dust samples were collected from the two elementary schools. Trace metal elements were extracted from the dust using hydrochloric acid and nitric acid, and the amounts were measured by ICP-OES.
Microbiological analysis was performed by bacterial culturing using R2A medium and denaturing gradient gel electrophoresis (DGGE).
Results: The results showed that the amounts of three metal elements (Cr, Pb, and Zn) were significantly different between the schools (¥á=0.05, p<0.04). In addition, microbial communities in each school were highly correlated with one another. Among the identified microorganisms, a number of potentially opportunistic microorganisms, including antibiotic-resistant bacteria such as Acinetobacter baumannii, were found.
Conclusions: This study will provide preliminary data for assessing levels and types of chemical and microbiological agents in elementary schools and for further evaluating human health risks associated with the agents.
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KEYWORD
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Bacterial antibiotic resistance, Denaturing gradient gel electrophoresis, Dust, Heavy metals, Shihwa industrial complex
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